K. Seed

The vibriophage-encoded inhibitor OrbA abrogates BREX-mediated defense through the ATPase BrxC

This paper identifies how the phage-encoded inhibitor OrbA disrupts the BREX defense system in Vibrio cholerae by directly binding to the BrxC ATPase component.

A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread

This study identifies Gpi, a PLE-encoded inhibitor of ICP1's DNA packaging motor, and shows how PLEs use multiple strategies to overcome inhibition and promote their own spread.

Sporadic phage defense in epidemic Vibrio cholerae mediated by the toxin-antitoxin system DarTG is countered by a phage-encoded antitoxin mimic

The paper identifies the DarTG toxin-antitoxin system in Vibrio cholerae that inhibits the lytic phage ICP1, and shows ICP1 counters this with an antitoxin mimic, revealing co-evolutionary dynamics between the bacterium and its phage.

Adaptation to bile and anaerobicity limits Vibrio cholerae phage adsorption

This study shows that Vibrio cholerae reduces O1-antigen on its outer membrane in response to bile and anaerobic conditions, limiting phage adsorption by ICP1.

A phage weaponizes a satellite recombinase to subvert viral restriction

The paper identifies a phage-encoded protein, Adi, that subverts a bacterial restriction system by repurposing a recombinase to enhance nuclease activity against the restriction element.

The RNA-RNA interactome between a phage and its satellite virus reveals a small RNA differentially regulates gene expression across both genomes

Evolutionary sweeps of subviral parasites and their phage host bring unique parasite variants and disappearance of a phage CRISPR-Cas system

The paper identifies new PLE variants in V. cholerae and links their spread to the loss of a CRISPR-Cas system in its phage host.

A phage parasite deploys a nicking nuclease effector to inhibit replication of its viral host

This study identifies a phage-encoded nuclease, NixI, that inhibits replication of its viral host ICP1 by nicking its genome, preventing efficient packaging.

A Tail Fiber Protein and a Receptor-Binding Protein Mediate ICP2 Bacteriophage Interactions with Vibrio cholerae OmpU

This study identifies ICP2 phage proteins involved in binding to V. cholerae OmpU and shows that mutations in gp25 and gp23 are required for host-range adaptation.

A chimeric nuclease substitutes CRISPR-Cas: A phage weaponizes laterally acquired specificity to destroy subviral parasites

The paper identifies a chimeric endonuclease in bacteriophage ICP1 that provides immunity against PLE mobile elements by cleaving their replication origins.

A phage satellite tunes inducing phage gene expression using a domesticated endonuclease to balance inhibition and virion hijacking

This study reveals how the phage satellite PLE in Vibrio cholerae uses a domesticated endonuclease to regulate ICP1 gene expression, balancing inhibition and virion hijacking.

A family of viral satellites manipulates invading virus gene expression and affects cholera toxin mobilization

This study uses RNA-seq to analyze how viral satellites (PLEs) affect gene expression of their host phage ICP1 and the cholera toxin encoding phage CTX{phi} during infection.

Transcription Termination and Antitermination of Bacterial CRISPR Arrays

The paper investigates how Rho-dependent transcription termination affects CRISPR array length and identifies an antitermination mechanism that enables full transcription of CRISPR arrays.

Dominant Vibrio cholerae phage exhibits lysis inhibition sensitive to disruption by a defensive phage satellite

The paper identifies a phage satellite gene that disrupts lysis inhibition in Vibrio cholerae, limiting phage progeny and affecting evolutionary dynamics.

Viral satellites exploit phage proteins to escape degradation of the bacterial host chromosome

The paper shows that phage satellites like PLE in Vibrio cholerae use phage-encoded helicases to replicate and evade phage-mediated genome degradation.

Genome replication dynamics of a bacteriophage and its satellite reveal strategies for parasitism and viral restriction

The paper investigates how PLE DNA replication interferes with ICP1 phage replication in Vibrio cholerae, revealing that replication is one of multiple mechanisms for viral restriction.

Competition between mobile genetic elements drives optimization of a phage-encoded CRISPR-Cas system: Insights from a natural arms-race

The paper studies CRISPR-Cas spacer acquisition and interference in a phage-against-mobile-genetic-element arms race, finding that chromosomal spacers can block PLE mobilization.

Anti-phage islands force their target phage to directly mediate island excision and spread

This study identifies a unique recombination system in V. cholerae PLEs that uses a phage-encoded protein to mediate excision in response to ICP1 infection.