BiochemistrybioRxivPreprint
In vitro reconstitution of vertebrate Sonic Hedgehog protein cholesterolysis
Dayton C. Seidel, Andrew G. Wagner, John L. Pezzullo…·Brian P. Callahan
This study establishes an in vitro FRET-based assay to monitor cholesterolysis activity of vertebrate SHhC domains, revealing substrate specificity and identifying a conserved aspartate residue critical for activity.