Validated CRISPR/Cas9 guide RNAs targeting neurodevelopmental genes in the tunicate Ciona robusta
Sydney Popsuj, Tenzin Kalsang, Kwantae Kim…·Alberto Stolfi
The paper validates CRISPR/Cas9 guide RNAs for eight neurodevelopmental genes in Ciona robusta, showing varying mutagenesis efficacy and improved correlation with RS3 scores.