Uncoupling of the Diurnal Growth Program by Artificial Genome Relaxation in Synechocystis sp. PCC 6803
A. Behle, M. Dietsch, L. Goldschmidt, W. Murugathas, D. Brandt, T. Busche, J. Kalinowski, O. Ebenhoeh, I. M. Axmann, R. Machne
The paper manipulates DNA supercoiling in Synechocystis sp. PCC 6803 using CRISPRi and TopoI overexpression, revealing its role in diurnal gene regulation and growth.
bioRxiv · Jul 26, 2021 · PreprintMicrobiology