M. L. Donnelly

This study develops a dual fluorescent reporter system to analyze toxin and sporulation gene expression in C. difficile, revealing a division of labor between virulence and transmission gene expression under specific growth conditions.

This study investigates whether restoring the catalytic triad in C. difficile pseudoproteases CspC and CspA can revive their protease activity during spore germination, finding that catalytic triad presence does not guarantee protease function.

This study identifies CspC as a bile salt sensor for C. difficile spore germination and shows it also responds to amino acid and calcium co-germinants through specific residues.