The paper introduces a solid-phase extraction method using metal oxide-coated silica columns to fractionate lipids into neutral, phospholipids, and other lipid classes for lipidomics.

bioRxiv · Oct 15, 2023Bioengineering

Multicellular dynamics on structured surfaces: Stress concentration is a key to controlling complex microtissue morphology on engineered scaffolds

R. Matsuzawa, A. Matsuo, S. Fukamachi, S. Shimada, M. Takeuchi, T. Nishina, P. Kollmannsberger, R. Sudo, S. Okuda, T. Yamashita

This study identifies stress concentration as a key factor in microtissue detachment from engineered scaffolds and develops a computational model to predict and control this process.

bioRxiv · Mar 6, 2023Biophysics

Characterisation of an Escherichia coli line that completely lacks ribonucleotide reduction yields insights into the evolution of obligate intracellularity.

S. D. M. Arras, N. Sibaeva, R. J. Catchpole, N. Horinouchi, D. Si, A. M. Rickerby, K. Deguchi, M. Hibi, K. Tanaka, M. Takeuchi, J. Ogawa, A. M. Poole

This paper creates an E. coli strain lacking ribonucleotide reduction and shows it adapts to depend on host-derived deoxyribonucleotides, mimicking evolutionary steps in obligate intracellular life.

bioRxiv · Sep 28, 2022Microbiology

The Intestinal Microbiome, Dietary Habits, and Physical and Psychological Resilience in Postpartum Women

M. Matsunaga, M. Takeuchi, S. Watanabe, A. K. Takeda, T. Kikusui, K. Mogi, M. Nagasawa, K. Hagihara, M. Myowa

This study examines the relationship between intestinal microbiota, physical condition, and psychological resilience in postpartum Japanese women, finding links between specific microbial genera and mental health risks.

bioRxiv · Sep 7, 2022 · PreprintMicrobiology

Synergy of turbulence and fishing reduce aquatic biomass

J. C. McKerral, J. R. Seymour, T. J. Lavery, P. J. Rogers, T. C. Jeffries, J. S. Paterson, B. Roudnew, C. Huveneers, K. Newton, V. van Dongen-Vogels, N. P. Cribb, K. M. Winn, R. J. Smith, C. L. Beckmann, E. Prime, , J. G. Mitchell

The paper shows that ocean turbulence and fishing both reduce aquatic biomass, with turbulence having a larger impact by increasing the energetic cost of movement for nekton.

bioRxiv · Oct 4, 2021 · PreprintEcology

Lipopolysaccharide stimulation of RAW264.7 cells is a model for identifying novel clients of Hsc70

J. F. Rakus, N. R. Kegley, A. J. Ashley, M. A. Parsons, M. Takeuchi

The paper identifies novel Hsc70 protein clients using LPS-stimulated RAW264.7 cells through immunoprecipitation and mass spectrometry, finding 34 LPS-specific interactions.

bioRxiv · May 15, 2020 · PreprintBiochemistry

Subcellular localization of glypican-5 associates with dynamic cell motility and cell communication of the human mesenchymal stem cell line U3DT

M. Takeuchi, K. Takeuchi, Y. Monobe, T. Takai, R. Yamaguchi, J. K. Takeuchi, K.-i. Akagi

The paper investigates the subcellular localization of GPC5 in U3DT cells and links its distribution to cell motility and communication.

bioRxiv · Dec 7, 2019Cell Biology