D. Si

Gradual labeling with fluorogenic probes for MINFLUX nanoscopy of densely packed structures

This paper introduces GLF-MINFLUX, a method for nanoscale imaging of densely packed structures, achieving improved localization precision and faster acquisition.

PerturBase: a comprehensive database for single-cell perturbation data analysis and visualization

PerturBase is a database that compiles and visualizes single-cell perturbation data from multiple studies, enabling analysis of genetic and chemical perturbations across millions of cells.

Characterisation of an Escherichia coli line that completely lacks ribonucleotide reduction yields insights into the evolution of obligate intracellularity.

This paper creates an E. coli strain lacking ribonucleotide reduction and shows it adapts to depend on host-derived deoxyribonucleotides, mimicking evolutionary steps in obligate intracellular life.

Structure of Geobacter OmcZ filaments suggests extracellular cytochrome polymers evolved independently multiple times

The paper determines the structure of OmcZ filaments in Geobacter sulfurreducens, showing they have a distinct heme arrangement compared to OmcS and OmcE, suggesting independent evolution of conductive cytochrome polymers.

DeepTracer ID: De Novo Protein Identification from Cryo-EM Maps

DeepTracer-ID identifies proteins from cryo-EM maps without additional sequence information by combining DeepTracer modeling with AlphaFold2 predictions.

Structural Insights Into the Initiation and Elongation of Ubiquitination by Ubr1

This study reports cryo-EM structures of Ubr1 complexes to elucidate the mechanism of ubiquitination initiation and elongation using a single E2 enzyme.

Outcomes of the 2019 EMDataResource model challenge: validation of cryo-EM models at near-atomic resolution

The paper evaluates cryo-EM model quality using benchmark maps, showing high accuracy and reproducibility across 13 teams and recommending multiple metrics for model assessment.

Toward Broad Spectrum DHFR inhibitors Targeting Trimethoprim Resistant Enzymes Identified in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

The paper develops novel inhibitors targeting DHFR enzymes in MRSA to overcome trimethoprim resistance, showing low nanomolar activity and human selectivity.