MicrobiologyMicrobiologyPeer-reviewed
Culturomics from field-grown crop plants using dilution-to-extinction, two-step library preparation and amplicon sequencing
Eglantina Lopez‐Echartea, Nicholas S. Dusek, Mallory Misialek…·Barney A. Geddes
This paper optimizes a culturomics protocol to isolate and identify hundreds of bacterial isolates from plant microbiomes using dilution-to-extinction culturing and a two-step barcoding PCR strategy with NGS and DADA2.
4 citations