Synthetic BiologyMicrobial BiotechnologyPeer-reviewed
Integrating continuous hypermutation with high-throughput screening for optimization of cis,cis-muconic acid production in yeast
Emil D. Jensen, Francesca Ambri, Marie B. Bendtsen…·Jay D. Keasling
The paper develops a directed evolution workflow combining OrthoRep and a transcription factor-based biosensor to optimize cis,cis-muconic acid production in yeast, achieving >13-fold higher titers.
40 citations