Author

Mark Gabriel S. Sagarbarria

University of the Philippines Los Baños

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Molecular Biology· 1 Plant Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:plant (other)· 2 Research Focus:benchmarking· 1 Assay:CRISPR screen· 1

Frequent Co-Authors

John Albert M. CaraaniD· 2 shared Angelo John Gilbuena Layos· 1 shared Desiree M. HauteaiD· 1 shared Ian Bien M. Oloc-oloc· 1 shared Kazuo WatanabeiD· 1 shared Patrick Louie G. Lipio· 1 shared

Activity by Year

2023-2024 · peak 1/yr

Co-Author Collaboration Network

6 top collaborators · 11 mutual co-author links

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2 shared papersPlant Biology

John Albert M. CaraanORCID

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2 papers

Recent·Most cited

Plant BiologyThe Philippine Agricultural ScientistPeer-reviewedMar 1, 2024

Agrobacterium-mediated Genetic Transformation and Plant Regeneration from Cotyledons in Philippine Eggplant (Solanum melongena L.) Acc. "PH 11424"

Mark Gabriel S. Sagarbarria, John Albert M. Caraan, Patrick Louie G. Lipio…·Desiree M. Hautea

This study establishes an Agrobacterium-mediated genetic transformation and plant regeneration protocol for a Philippine eggplant cultivar using cotyledons as explants, achieving 5.1% regeneration efficiency.

2 citations

plant (other)experimental assay

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Molecular BiologyTransgenic ResearchPeer-reviewedOct 24, 2023

Usefulness of Current sgRNA Design Guidelines and in vitro Cleavage Assays for Plant CRISPR/Cas Genome Editing: A Case in Eggplant (Solanum melongena L.)

Mark Gabriel S. Sagarbarria, John Albert M. Caraan, Angelo John Gilbuena Layos

The paper evaluates sgRNA design guidelines and in vitro assays for plant CRISPR/Cas9, finding that in vitro cleavage does not reliably predict in vivo efficiency and highlights the need for multiple high-performing sgRNAs.

13 citations

plant (other)CRISPR screen

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