GeneticsbioRxiv
In Vivo Blunt-End Cloning Through CRISPR/Cas9-Facilitated Non-Homologous End-Joining
Jonathan M Geisinger, Sören Turan, Sophia Hernandez…·Michele P Calos
This paper introduces an in vivo blunt-end cloning method using CRISPR/Cas9 to insert exogenous sequences with high efficiency in human cells.
DOI 10.1093/nar/gkv1542