BiochemistryAnalytical BiochemistryPeer-reviewed
In-gel refolding allows fluorescence detection of fully denatured GFPs after SDS-PAGE
Misa Shiratori, Rio Tsuyuki, Miwako Asanuma…·Kenji Ohgane
This paper introduces a method to refold fully denatured GFPs within SDS-PAGE gels using cyclodextrin and methanol, enabling in-gel fluorescence detection.