Systematic characterization of existing and novel inducible transgenic systems in human pluripotent stem cells after prolonged differentiation
Michael D. Gallagher, Andrew S. Khalil, Qi Liu, Tenzin Lungjangwa, Antar Drews, Blake F. Hanan, Moritz List, Henry Hardart, David J. Mooney, Rudolf Jaenisch
This study evaluates inducible transgenic systems in human pluripotent stem cells and differentiated cells, identifying a small molecule-mediated splicing switch that enables tunable transgene expression without protein tags.
bioRxiv · Oct 17, 2025 · PreprintBioengineering