N. Boon

Molybdate delays sulphide formation in the sediment and transfer to the bulk liquid in a model shrimp pond

This study shows that molybdate treatment reduces sulphide production in shrimp pond sediments by altering microbial communities and delaying sulphide transfer to the bulk liquid.

Unlocking the Mechanism of Action: A Cost-Effective Flow Cytometry Approach for Accelerating Antimicrobial Drug Development

This paper introduces a cost-effective flow cytometry approach to predict antimicrobial mechanisms of action using random forest classifiers trained on phenotypic fingerprints.

Impact of operational conditions on drinking water biofilm dynamics and coliform invasion potential

This study investigates how operational changes affect drinking water biofilm dynamics and their susceptibility to coliform invasion, finding that mature biofilms can be infiltrated by low concentrations of Serratia fonticola.

mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene therapy

This paper introduces REVeRT, a dual AAV vector system using mRNA trans-splicing for efficient gene delivery and editing, showing improved reconstitution and therapeutic potential in a mouse model.

Real-Time Flow Cytometry to assess qualitative and quantitative responses of oral pathobionts during exposure to antiseptics

This study uses real-time flow cytometry to rapidly assess the quantitative and qualitative responses of oral bacteria to antiseptics and identifies treatment-specific phenotypic subpopulations.

Triangulation of microbial fingerprinting in anaerobic digestion reveals consistent fingerprinting profiles

This study compares four microbial fingerprinting methods in anaerobic digestion, finding that cytometric fingerprinting via flow cytometry provides reliable and fast holistic monitoring, while other methods offer complementary insights.

Predicting the presence and abundance of bacterial taxa in environmental communities through flow cytometric fingerprinting

The paper develops a computational pipeline using flow cytometry and 16S rRNA gene amplicon sequencing to predict bacterial taxa presence and abundance in environmental communities.

Selective metal extraction by biologically produced siderophores during bioleaching from low-grade primary and secondary mineral resources

This study evaluates siderophore-mediated metal extraction from mineral resources, finding that purified PyoPpC-3B can selectively chelate Mn2+ and Zn2+ over Fe3+.

Rearing water microbiomes in white leg shrimp (Litopenaeus vannamei) larviculture assemble stochastically and are influenced by the microbiomes of live feed products

This study examines the stochastic assembly of rearing water microbiomes in shrimp larviculture and quantifies the contribution of live and dry feed microbiomes to these communities.

PhenoGMM: Gaussian mixture modelling of microbial cytometry data enables efficient predictions of biodiversity

PhenoGMM uses Gaussian Mixture Models to predict microbial diversity from flow cytometry data, integrating with 16S rRNA sequencing for improved accuracy.

Cytometric fingerprints of gut microbiota predict Crohn’s disease state

The paper shows that flow cytometry can classify Crohn’s disease samples based on gut microbiota changes, matching 16S rRNA gene analysis results.

Discriminating bacterial phenotypes at the population and single-cell level: a comparison of flow cytometry and Raman spectroscopy fingerprinting

This paper compares flow cytometry and Raman spectroscopy for analyzing bacterial phenotypic heterogeneity, showing flow cytometry's population-level resolution and Raman's single-cell biochemical detail.

Randomized lasso associates freshwater lake-system specific bacterial taxa with heterotrophic production through flow cytometry

The paper uses machine learning to link bacterial taxa with heterotrophic production in freshwater lakes using flow cytometry and 16S rRNA data.

Coculturing bacteria leads to reduced phenotypic heterogeneities

This study shows that coculturing bacteria reduces their individual phenotypic heterogeneity, suggesting that community composition influences taxon-specific diversity.