SILAKin: A novel high throughput SILAC and mass spectrometry-based assay to identify the substratome of kinases secreted by pathogens
D. Smirlis, F. Dingli, V. Sabatet, A. Roth, U. Knippchild, D. Loew, G. F. Spaeth, N. Rachidi
SILAkin identifies kinase substrates using a combination of phosphatase treatment, pulse heating, IVKA, and SILAC-based MS, revealing 225 host substrates phosphorylated by pathogen-excreted kinases.
bioRxiv · May 5, 2021Biochemistry