D. Loew

A multi-year longitudinal harmonization of quality controls in mass spectrometry proteomics core facilities

This study harmonizes quality controls across proteomics core facilities to improve data comparability and reliability through a 4-year longitudinal dataset and common processing software.

Inhibition of the macrophage demethylase LSD1 reverses Leishmania amazonensis-induced transcriptomic changes and causes a decrease in parasite load

This study shows that inhibiting LSD1 in macrophages reduces Leishmania parasite load and reverses infection-induced transcriptomic changes.

Growth factor-induced desialylation for the fast control of endocytosis

The paper shows that growth factors induce rapid desialylation of cell surface glycoproteins, enabling reversible changes in glycan structure and function through galectin-mediated endocytosis.

RNA polymerase III is involved in regulating Plasmodium falciparum virulence

The paper shows RNA polymerase III regulates Plasmodium falciparum virulence by controlling cytoadhesion and responding to external stimuli in asymptomatic infections.

Identification of the Intracellular Protein Targets of a Bio-active Clickable Half-Sandwich Iridium Complex by Chemical Proteomics

This study identifies intracellular protein targets of a clickable iridium complex using chemical proteomics, revealing dual electrophilic and oxidative stress mechanisms.

Polθ is phosphorylated by Polo-like kinase 1 (PLK1) to enable repair of DNA double strand breaks in mitosis

This study identifies Polθ as a key factor in repairing DNA double strand breaks during mitosis, showing that its phosphorylation by PLK1 enables this process and maintains genome integrity.

Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to identify their phospho-dependent binding partners.

The paper structurally characterizes disordered domains of pluripotency transcription factors and presents a method to identify their phospho-dependent binding partners using NMR and proteomics.

The functional specificity of CDC42 isoforms is caused by their distinct sub cellular localization

This study shows that CDC42 isoforms differ in subcellular localization, which determines their distinct roles in cell polarization and endocytosis.

The oncogenic role of Streptococcus gallolyticus subsp. gallolyticus is linked to activation of multiple cancer-related signaling pathways

This study shows that SGG UCN34 promotes colon cancer by activating multiple cancer-related signaling pathways through proteomic and phosphoproteomic analyses in mice and human tumors.

Extracellular vesicles and co-isolated endogenous retroviruses differently affect dendritic cells

The paper identifies virus-like particles in tumor-derived extracellular vesicles and shows they differentially affect dendritic cells compared to other nanoparticles.

Atheroprone shear stress stimulates noxious endothelial extracellular vesicle uptake by MCAM and PECAM-1 cell adhesion molecules

This study investigates how atheroprone and atheroprotective shear stress affects endothelial extracellular vesicle (EV) release and uptake, finding that LSS increases EV uptake through adhesion molecules like MCAM and PECAM-1.

Fibroblasts generate topographical cues that steer cancer cell migration

This study shows fibroblasts create tubular structures that guide cancer cell migration by acting as physical tracks.

SILAKin: A novel high throughput SILAC and mass spectrometry-based assay to identify the substratome of kinases secreted by pathogens

SILAkin identifies kinase substrates using a combination of phosphatase treatment, pulse heating, IVKA, and SILAC-based MS, revealing 225 host substrates phosphorylated by pathogen-excreted kinases.

Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme

This study identifies THBS1-YAP signaling as a paracrine mechanism that reprograms healthy intestinal epithelial cells to support tumor growth.

Specificities of exosome versus small ectosome secretion revealed by live intracellulartracking and synchronized extracellular vesicle release of CD9 and CD63

This study compares exosome and small ectosome secretion in HeLa cells using live tracking and proteomic analysis, revealing differences in their trafficking and release mechanisms.

Microtubules tune mechanosensitive cell responses

This study shows that microtubule acetylation, regulated by actomyosin mechanosensing, influences RhoA activation and cell migration.

Genome wide natural variation of H3K27me3 selectively marks genes predicted to be important for cell differentiation in Phaeodactylum tricornutum

The paper shows that H3K27me3, deposited by PRC2, is involved in cell differentiation in the unicellular diatom Phaeodactylum tricornutum.

Surface LSP1 is a phenotypic marker distinguishing human classical versus monocyte-derived dendritic cells

CD44 regulates epigenetic plasticity by mediating iron endocytosis

This study identifies CD44 as a mediator of iron endocytosis linked to epigenetic plasticity, showing iron's role in regulating histone modifications and gene expression during mesenchymal transitions.

Arabidopsis S2Lb links AtCOMPASS-like and SDG2 activity in histone H3 Lys-4 trimethylation independently from histone H2B monoubiquitination

This study identifies S2Lb as a co-regulator of H3K4me3 with SDG2 in Arabidopsis, showing H3K4me3 deposition is H2Bub-independent and important for gene induction during cellular transitions.

DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis

The paper shows DET1 regulates H2Bub homeostasis in Arabidopsis by controlling the stability of a DUBm through interaction with DDA1.