GenomicsNucleic Acids ResearchPeer-reviewed
Metagenomics vs. total RNA sequencing: most accurate data-processing tools, microbial identification accuracy, and implications for freshwater assessments
Christopher A. Hempel, Natalie Wright, Julia Harvie…·Dirk Steinke
This study compares metagenomics and total RNA-Seq for microbial identification accuracy using 768 workflows, finding total RNA-Seq more accurate at lower sequencing depths and suggesting its potential for cost-effective freshwater assessments.
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