Oliver Takacsi-Nagy

This paper identifies methods to achieve ultra-large DNA integrations (>10 kb) in human cells with high efficiency using optimized templates and delivery strategies.

The paper identifies off-target effects in CRISPRi Perturb-seq experiments by analyzing transcriptional similarity between on-target and off-target guide effects.

The paper uses Molecular Pixelation to analyze surface proteomic signatures of CAR T cells under acute and chronic stimulation, identifying spatial patterns linked to exhaustion.

CRISPR-All enables genome-scale, combinatorial genetic perturbations in human cells, revealing additive functional improvements through multiplexed modifications.

The paper develops and applies a scalable method to map transcription factor binding in human T cells, revealing novel regulatory dynamics and gene targets.

This paper introduces non-viral intron targeting to improve genetic editing flexibility and simplify cell selection by integrating synthetic exons into endogenous introns.

This paper designs novel transcription factors using evolutionary principles to generate T cell states with enhanced functionality.

The paper investigates the role of H2A subtypes in CAG/CTG repeat instability in yeast, finding H2A.1 protects against expansion and its phosphorylation at Thr126 is critical for repair fidelity.