Dual-specific autophosphorylation of kinase IKK2 enables phosphorylation of substrate IκBα through a phosphoenzyme intermediate
P. Borar, T. Biswas, A. Chaudhuri, P. T. Rao, S. Raychaudhuri, T. Huxford, S. Chakrabarti, G. Ghosh, S. Polley
This study identifies dual-specific autophosphorylation in IKK2, showing tyrosine autophosphorylation and ATP-contacting residue K44 regulate substrate specificity for IκBα phosphorylation during NF-κB activation.
eLife · Jun 30, 2025Peer-reviewedBiochemistry