Author

S. Polley

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biochemistry· 5 Cell Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 5 Research Focus:cell signaling & trafficking· 3 Organism:human· 2 Assay:proteomics· 2 Research Focus:cell type annotation· 1 Research Focus:chromatin & epigenetic regulation· 1 Approach:deep learning· 1 Research Focus:development & morphogenesis· 1

Frequent Co-Authors

A. Musacchio· 2 shared G. Ghosh· 2 shared T. Biswas· 2 shared A. Alex· 1 shared A. Chaudhuri· 1 shared A. De Antoni· 1 shared A. Petrovic· 1 shared B. Voss· 1 shared

Activity by Year

2019-2025 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 12 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

2 shared papersBiochemistry

A. Musacchio

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Dual-specific autophosphorylation of kinase IKK2 enables phosphorylation of substrate IκBα through a phosphoenzyme intermediate

P. Borar, T. Biswas, A. Chaudhuri, P. T. Rao, S. Raychaudhuri, T. Huxford, S. Chakrabarti, G. Ghosh, S. Polley

This study identifies dual-specific autophosphorylation in IKK2, showing tyrosine autophosphorylation and ATP-contacting residue K44 regulate substrate specificity for IκBα phosphorylation during NF-κB activation.

eLife · Jun 30, 2025Peer-reviewedBiochemistry

Stable kinetochore-microtubule attachment requires loop-dependent Ndc80-Ndc80 binding

S. Polley, H. Müschenborn, M. Terbeck, A. De Antoni, I. R. Vetter, M. Dogterom, A. Musacchio, V. A. Volkov, P. J. Huis in 't Veld

This study shows that the Ndc80 loop facilitates Ndc80-Ndc80 interactions, which are essential for stable kinetochore-microtubule attachments and proper chromosome congression.

bioRxiv · Aug 25, 2022Biochemistry

Discovery of an IKK2 Site that Allosterically Controls Its Activation

S. J. Hotchkiss, M. C. Mulero, G. J. Chan, T. Biswas, S. Polley, C. Ohn, S. Konduri, O. Demir, R. E. Amaro, G. Ghosh

This paper identifies a novel allosteric site on IKK2 that can be targeted by small-molecule inhibitors to block its activity without ATP-competitive off-target effects.

bioRxiv · Jan 27, 2021 · PreprintBiochemistry

Electroporated recombinant proteins as tools for in vivo functional complementation, imaging, and chemical biology

A. Alex, V. Piano, S. Polley, M. Stuiver, S. Voss, G. Ciossani, K. Overlack, B. Voss, S. Wohlgemuth, A. Petrovic, Y.-W. Wu, P. Selenko, A. Musacchio, S. Maffini

This study demonstrates that electroporation can deliver recombinant proteins into human cells, maintaining their localization, function, and interactions, making it a versatile tool for chemical and synthetic biology.

bioRxiv · May 8, 2019Cell Biology

Indirect Readout of DNA Controls Filamentation and Activation of a Sequence-Specific Endonuclease

S. Polley, D. Lyumkis, N. C. Horton

This study reveals how SgrAI's filamentation and indirect DNA readout enhance its DNA cleavage activity and sequence specificity.

bioRxiv · Mar 22, 2019Biochemistry

A staphylococcal cyclophilin carries a single domain and unfolds via the formation of an intermediate that preserves cyclosporin A binding activity

S. Seal, S. Polley, S. Sau

This study investigates the unfolding mechanism and domain structure of a staphylococcal cyclophilin, revealing a single domain with a flexible tail and reversible unfolding via an intermediate that retains cyclosporin A binding activity.

bioRxiv · Jan 3, 2019Biochemistry