Exposing the molecular heterogeneity of glycosylated biotherapeutics
L. F. Schachner, C. Mullen, W. Phung, J. D. Hinkle, M. I. Beardsley, T. Bentley, P. Day, C. Tsai, S. Sukumaran, T. Baginski, D. DiCara, N. Agard, M. Masureel, J. Gober, A. ElSohly, , W. Sandoval
This paper introduces a new method for analyzing glycoform heterogeneity in biotherapeutics using mass spectrometry and bioinformatics, showing its effectiveness in measuring proteoform diversity and correlating glycoform sub-populations with pharmacological properties.
bioRxiv · May 10, 2023Biochemistry