Monoclonal antibody stability can be usefully monitored using the excitation-energy-dependent fluorescence edge-shift
M. Knight, R. Woolley, A. Kwok, S. Parsons, H. Jones, C. Gulacsy, P. Phaal, O. Kassaar, K. Dawkins, E. Rodriguez, A. Marques, L. Bowsher, S. A. Wells, A. Watts, J. M. van den Elsen, , C. R. Pudney
This paper demonstrates that quantifying the fluorescence red edge excitation shift (REES) can sensitively monitor monoclonal antibody stability across timeframes and concentrations.
bioRxiv · Mar 23, 2020Biochemistry