BiochemistrybioRxivPreprint
Expression Strategies for Recombinant HECT E3 Ligases in Escherichia coli
Paulina Krach, Daria Gajewska, Maria Sagan…·Michał J. Walczak
This study compares the expression of three HECT E3 ligases in E. coli, finding that lower temperatures improve solubility and yield, while full-length proteins are less successfully expressed than isolated domains.
DOI 10.1101/2023.12.28.573528