Peter J. Colias

The paper shows RNA m6A modifications coordinate with de novo DNA methylation to establish heritable epigenetic silencing in human cells.

The paper identifies regulators of microglial activation states using CRISPR screens and single-cell transcriptomics, revealing functional roles of ZNF532, PRDM1, and DNMT1 in disease-associated microglial states.

CRISPRtune is a programmable tool that tunes transcriptional repression by fusing MeCP2 to dCas9, enabling intermediate gene expression control in human cells.

LEMONmethyl-seq profiles DNA methylation dynamics in mammalian cells, revealing CRISPR-induced changes and endogenous patterns, including non-canonical CpH methylation.

This paper introduces RENDER, a method for transient delivery of CRISPR-based epigenome editors as ribonucleoproteins to modulate gene expression in human cells, achieving durable epigenetic silencing.