BioengineeringbioRxiv
Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
D. J. Knapp, Y. S. Michaels, M. Jamilly…·T. A. Fulga
The paper identifies sequence determinants of tRNA promoter and processing activities to engineer a Pol-II promoter for specific guide RNA expression.
DOI 10.1038/s41467-019-09148-3