Author

R. A. Marklein

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 5

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Research Focus:cell type annotation· 5 Organism:human· 5 Assay:proteomics· 4 Research Focus:drug response / drug discovery· 2 Approach:experimental assay· 2 Approach:statistical model· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:cell-cell communication· 1

Frequent Co-Authors

S. H. Andrews· 3 shared A. M. Larey· 2 shared H. M. Hynds· 2 shared K. M. Hines· 2 shared K. R. Daga· 2 shared M. G. Morfin· 2 shared S. L. Stice· 2 shared A. O. A. Michael· 1 shared

Activity by Year

2020-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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3 shared papersBioengineering

S. H. Andrews

Mutual co-authors in cluster

Author profile →

5 papers

Recent·Most cited

Microglia Morphological Response to Mesenchymal Stromal Cell Extracellular Vesicles Demonstrates EV Therapeutic Potential for Modulating Neuroinflammation

K. R. Daga, A. M. Larey, M. G. Morfin, K. Chen, S. Bitarafan, J. M. Carpenter, H. M. Hynds, K. M. Hines, L. B. Wood, R. A. Marklein

This paper demonstrates that MSC-EVs modulate microglia morphology and cytokine secretion, suggesting their therapeutic potential for neuroinflammation, and introduces a high-throughput morphological screening method for EV bioactivity.

bioRxiv · Jul 1, 2024Bioengineering

High throughput screening of mesenchymal stromal cell morphological response to inflammatory signals for bioreactor-based manufacturing of extracellular vesicles that modulate microglia

A. M. Larey, T. M. Spoerer, K. R. Daga, M. G. Morfin, H. M. Hynds, J. Carpenter, K. M. Hines, R. A. Marklein

The paper screens MSC morphology in response to inflammatory priming to identify optimal conditions for EV production and microglia modulation in bioreactors.

bioRxiv · Nov 19, 2023Bioengineering

Multi-omics characterization of mesenchymal stem/stromal cells for the identification of putative critical quality attributes

T. S. Maughon, X. Shen, D. Huang, A. O. A. Michael, W. A. Shockey, S. H. Andrews, J. M. McRae, M. O. Platt, F. M. Fernandez, A. S. Edison, S. L. Stice, R. A. Marklein

This paper identifies putative critical quality attributes (CQAs) for mesenchymal stem/stromal cells (MSCs) using multi-omics data and PLSR modeling to predict functional capacity.

bioRxiv · May 10, 2021 · PreprintBioengineering

Morphological Landscapes from High Content Imaging Identify Optimal Priming Strategies that Enhance MSC Immunosuppression

S. H. Andrews, M. W. Klinker, S. R. Bauer, R. A. Marklein

This study uses high-content imaging to analyze MSC morphology under different priming conditions and finds that combined IFN-γ/TNF- priming reduces functional heterogeneity and enhances immunosuppression.

bioRxiv · Feb 23, 2021 · PreprintBioengineering

Priming of MSCs with inflammation-relevant signals affects extracellular vesicle biogenesis, surface markers, and modulation of T cell subsets

S. H. Andrews, T. Maughon, R. A. Marklein, S. L. Stice

This study shows that acidosis-primed MSCs produce EVs that enhance regulatory T cell frequencies and uptake, suggesting acidosis could improve MSC-EV therapeutic potential.

bioRxiv · Apr 30, 2020Bioengineering