Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis/release regulators
K. Kunitake, T. Mizuno, K. Hattori, C. Oneyama, M. Kamiya, S. Ota, Y. Urano, R. Kojima
The paper introduces CIBER, a CRISPR-based method to barcode sEVs for subpopulation-specific analysis of their release regulators, identifying new regulators and characterizing sEV biogenesis.
bioRxiv · Sep 28, 2023Cell Biology