Ruikai Jia

The paper introduces a method to filter abnormal modified base calling in Oxford Nanopore Technologies sequencing, improving accuracy and reducing the need for secondary sequencing.

This study evaluates PacBio sequencing for direct, full-length resolution of complex DNA sequences, finding it effective for high GC, poly, and repeat sequences like rAAV without pre-fragmentation.

This paper presents a method to improve PacBio sequencing of low-copy plasmids using Phi29 polymerase amplification and a plasmid-free sequencing approach.