Author

Ruixin Wang

Duke UniversityPrimary ORCID profile (0000-0003-2624-1593 · 2 papers)Unclaimed ORCID profile · follow to get notified of new preprints

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Ruixin WangiD2 papers · University of Kentucky

Biology Disciplines

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Synthetic Biology· 2

Signature Assays, Organisms & Methods

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Organism:E. coli· 2 Approach:experimental assay· 2 Research Focus:protein design· 2 Assay:proteomics· 1

Frequent Co-Authors

Eirik A. MorebiD· 2 shared John P. EfromsoniD· 2 shared Michael LynchiD· 2 shared Romel Menacho‐Melgar· 2 shared Tian YangiD· 2 shared Jennifer N. Hennigan· 1 shared John S. DeckeriD· 1 shared Zhixia Ye· 1 shared

Activity by Year

2020-2020 · peak 2/yr

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8 top collaborators · 26 mutual co-author links

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2 shared papersSynthetic Biology

Eirik A. MorebORCID

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2 papers

Recent·Most cited

Synthetic BiologyBiotechnology and BioengineeringPeer-reviewedMay 28, 2020

Improved, two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease

Romel Menacho‐Melgar, Eirik A. Moreb, John P. Efromson…·Michael Lynch

This paper describes a method to improve protein expression and purification in E. coli by inducing autolysis and DNA/RNA hydrolysis via phage lysozyme and endonuclease, achieving >90% protein release.

33 citations

E. coliproteomics

𝕏 PostBluesky

Synthetic BiologyBiotechnology and BioengineeringPeer-reviewedMay 22, 2020

Improved, scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.

Romel Menacho‐Melgar, Zhixia Ye, Eirik A. Moreb…·Michael Lynch

This paper describes an improved method for recombinant protein expression in E. coli using phosphate depletion to trigger autoinduction, achieving high protein titers across various scales.

44 citations

E. coliexperimental assay

𝕏 PostBluesky