Zhixia Ye

The paper develops 2-stage microfermentation protocols for high-throughput evaluation of engineered E. coli strains for producing various products.

This paper demonstrates that dynamic deregulation of central metabolism in E. coli improves process robustness and scalability for industrial chemical production.

This paper introduces a multi-agent directed evolution approach that identifies hidden mutations by using multiple substrates, improving enzyme engineering efficiency.

The paper shows that the Cas1/2 endonuclease complex in E. coli causes instability in CRISPR/Cascade guide arrays, and controlled expression of the Cascade operon improves stability.

This paper describes an improved method for recombinant protein expression in E. coli using phosphate depletion to trigger autoinduction, achieving high protein titers across various scales.

The paper tests the robustness of PhoB-regulated promoters in E. coli across different media and identifies promoters with consistent expression for two-stage autoinduction.