Author

Ryan T. Leenay

North Carolina State University

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Synthetic Biology· 2 Cell Biology· 1

Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 2 Approach:experimental assay· 2 Assay:whole-genome sequencing· 2 Assay:CRISPR screen· 1 Research Focus:drug response / drug discovery· 1 Organism:human· 1 Approach:machine learning (classical)· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

Chase L. BeiseliD· 2 shared Albert J. KeungiD· 1 shared Alexander MarsoniD· 1 shared Amirali Aghazadeh· 1 shared Andrew P. MayiD· 1 shared Chunyu LiaoiD· 1 shared David Tse· 1 shared Fani Ttofali· 1 shared

Activity by Year

2018-2018 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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2 shared papersSynthetic Biology

Chase L. BeiselORCID

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3 papers

Recent·Most cited

Cell BiologybioRxivPreprintAug 31, 2018

Systematic characterization of genome editing in primary T cells reveals proximal genomic insertions and enables machine learning prediction of CRISPR-Cas9 DNA repair outcomes

Ryan T. Leenay, Amirali Aghazadeh, Joseph Hiatt…·James Zou

This study systematically characterizes genome editing in primary T cells, identifies proximal genomic insertions, and develops a machine learning model to predict CRISPR-Cas9 DNA repair outcomes.

DOI 10.1101/404947

humanwhole-genome sequencing

𝕏 PostBluesky

Synthetic BiologybioRxivPreprintJun 20, 2018

Streamlined, recombinase-free genome editing with CRISPR-Cas9 in Lactobacillus plantarum reveals barriers to efficient editing

Ryan T. Leenay, Justin M. Vento, Malay Shah…·Chase L. Beisel

This study introduces a CRISPR-Cas9-based genome editing method for Lactobacillus plantarum without requiring recombinase, showing it can efficiently introduce stop codons and deletions but with some unexpected outcomes.

5 citations

bacteria (other)whole-genome sequencing

𝕏 PostBluesky

Synthetic BiologybioRxivPreprintMay 2, 2018

One-step assembly of large CRISPR arrays enables multi-functional targeting and reveals constraints on array design

Chunyu Liao, Fani Ttofali, Rebecca Slotkowski…·Chase L. Beisel

The paper introduces CRATES, a one-step method to assemble large CRISPR arrays, demonstrating its utility for multiplexed gene editing and revealing design constraints.

6 citations

bacteria (other)yeast

𝕏 PostBluesky