Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targeting
J. Wei, P. Lotfy, K. Faizi, S. Baungaard, E. Gibson, E. Wang, H. Slabodkin, E. Kinnaman, S. Chandrasekaran, H. Kitano, M. G. Durrant, C. V. Duffy, P. D. Hsu, S. Konermann
The paper develops a deep learning model to predict CRISPR-Cas13d guide RNA efficiency and identifies novel orthologs with improved specificity and reduced toxicity for RNA targeting.
bioRxiv · Sep 14, 2021Bioengineering