Computationally Engineered CRISPR-SpyCas9 High-Fidelity Variants with Improved Specificity and Reduced Non-specific DNA Damage
R. Rabinowitz, O. Shor, J. Zerbib, S. Herman, N. Zelikson, S. Madiwale, N. Yom-Tov, U. Ben-David, F. Benninger, D. Offen
The paper develops ComPE, a computational method to engineer high-fidelity CRISPR-Cas9 variants with improved specificity and reduced off-target effects.
bioRxiv · Apr 11, 2023 · PreprintBioengineering