Pairing two growth-based, high-throughput selections to fine tune conformational dynamics in oxygenase engineering
S. Maxel, L. Zhang, E. King, D. Aspacio, A. P. Acosta, R. Luo, H. Li
This paper introduces two growth-based selections in E. coli to engineer CHMO with improved thermostability and NADH cofactor specificity.
bioRxiv · May 22, 2020 · PreprintBioengineering