Takumi Iwasaka

This paper develops a drinking-water model of cholestatic liver injury in mice using MDA, showing sustained ALT elevation and distinct histological and biochemical features compared to TAA-DW.

This paper introduces a biosensor-based method for single-molecule enzyme activity profiling without enzyme-specific substrates, enabling absolute quantification of active enzyme molecules in cellular and circulating samples.

This study develops water-soluble fluorogenic probes for single-molecule glycosidase activity profiling in blood, revealing -mannosidase as a potential biomarker for liver injury.

This paper introduces a programmable chemical strategy called SCCR for automated synthesis of protease-activatable molecules, demonstrating its application in generating fluorogenic probes and ADC linkers.

This paper develops a single-molecule enzyme activity assay for serine hydrolases using activity-based probes and demonstrates its application in detecting active granzyme B as a biomarker of liver damage.