Molecular BiologyPhysiology and Molecular Biology of PlantsPeer-reviewed
Improved Validation of Protein Interactions usingBicistronic BiFC (Bi2FC)
Prakash Sivakumar, Vijayaraj Vaishnavi, Kothuri Gayatri…·Imran Siddiqi
The paper introduces an improved BiFC method for validating protein interactions in plants by incorporating bicistronic expression and self-cleaving peptides to better assess expression and interaction status.