MicrobiologybioRxiv
A new microfluidic device for simultaneous detection of enzyme secretion and elongation of a single hypha
A. Itani, Y. Shida, W. Ogasawara
This paper introduces a microfluidic device to observe single hyphae and detect enzyme secretion in real-time, showing a strong correlation between fluorescence signals and cellulase activity in Trichoderma reesei.
DOI 10.3389/fmicb.2023.1125760