Ye Bu

Precision DNA Impurity Reduction Approaches for Ultra-Pure rAAV Manufacturing

The paper evaluates DNA impurity reduction strategies in rAAV manufacturing, finding that TelN/TelROL and I-SceI reduce plasmid backbone DNA to 20-40%, CRISPR/Cas9 to 10-20%, and Cre/LoxP eliminates it without affecting vector titers.

Decoupling Efficacy from Toxicity: Engineering Spatial Control in AAV-Mediated Gene Therapy

This study shows that using tissue-specific AAV variants and local delivery reduces hepatic toxicity while maintaining therapeutic efficacy in gene therapy.

Extracellular matrix deposition precedes muscle-tendon integration during murine forelimb morphogenesis

The paper shows that an ECM template forms independently of muscle but requires muscle for proper MTJ-specific ECM assembly during limb development.

Engineering novel AAV capsids by global de-targeting and subsequent muscle-specific tropism in mice and NHPs

The paper develops AAV.eM, a muscle-specific rAAV capsid with reduced off-target transduction, showing efficacy in Duchenne Muscular Dystrophy models and favorable safety in mice and NHPs.

Quantitative imaging in whole-mount zebrafish embryos traces morphogen gradient maintenance and noise propagation in BMP signaling

The paper quantifies BMP signaling gradients in zebrafish embryos and shows how noise and Sizzled maintain the gradient during DV patterning.

WaveletSEG: Automatic wavelet-based 3D nuclei segmentation and analysis for multicellular embryo quantification

WaveletSEG provides a robust, automatic 3D nuclei segmentation method for multicellular embryo analysis without preprocessing, achieving high accuracy in noisy data.

Light-Sheet Fluorescence Imaging Charts the Gastrula Origin of Vascular Endothelial Cells in Early Zebrafish Embryos

This study uses light-sheet imaging to map the origin of vascular endothelial cells in zebrafish gastrulae, revealing contributions from different regions along developmental axes.