A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice.
Alexandr Klioutchnikov, Damian J. Wallace, Juergen Sawinski…·Jason N. D. Kerr
This paper describes a three-photon head-mounted microscope that enables imaging of all cortical layers in freely moving mice in fully lit environments, revealing differential neuronal activity in specific layers under different lighting conditions.