Ailong Ke

This paper reports cryo-EM structures of IscB bound to single-stranded nucleic acids, revealing a conformational checkpoint that regulates RNA targeting efficiency and introduces mutations to enhance it.

This study defines the DNA binding and cleavage mechanisms of Type I-C CRISPR-Cas3 using cryo-EM and identifies anti-CRISPR proteins that inhibit its activity in human cells.

The paper uses cryo-EM to reveal how TnsC regulates target-site selection in CRISPR-associated transposition systems by transferring polarity information to the transposase.