José M. Alonso

The paper develops ACE and ACE2 reporters to study hormone interactions in Arabidopsis roots, revealing spatial and temporal auxin-cytokinin-ethylene crosstalk mechanisms.

This study shows how ethylene biosynthesis shifts spatially during nodule initiation in Medicago truncatula, with specific ACS genes regulating infection and nodule formation.

The paper investigates the relationship between ascorbate levels and auxin biosynthesis in Arabidopsis by analyzing mutant lines with altered ascorbate concentrations.

This study shows that gene families involved in the IAOx pathway are not essential for auxin biosynthesis in Arabidopsis, challenging the proposed linear pathway and suggesting an uncharacterized route.

The paper develops and tests EBSn, a synthetic ethylene-inducible promoter that outperforms existing reporters in sensitivity and tissue coverage in Arabidopsis and tomato.

The paper develops a modular geminivirus-based vector system for improved protein expression and gene targeting in plants, showing that native promoters and removal of virion-sense genes enhance reporter expression without affecting replicon accumulation.

The paper introduces DASH, a gene stacking system for plant synthetic biology that overcomes limitations of existing methods by combining Golden Gate, recombineering, and recombinase systems.

This paper introduces append editing, a method that uses ADP-ribosylation to achieve distinct genome editing outcomes in bacteria and eukaryotes.

This study investigates MOR1's interaction with microtubule plus ends, showing its affinity for both growing and shrinking ends and its role in polymerization and depolymerization.

The paper identifies hypomorphic mutants of the ROOTY gene in plants and shows interallelic complementation in transheterozygotes, supported by structural modeling of RTY protein interactions.

The paper develops a recombineering-based toolset for high-throughput gene tagging in plants, enabling precise chromosomal modifications and efficient generation of transgenic lines.