Thomas Köhnke

The paper identifies and isolates human AML leukemia stem cells using single-cell multi-omics and establishes a prognostic gene signature for clinical relevance.

This study shows DNMT3AR882H mutations are not required for maintaining AML but are linked to higher leukemia stem cell frequency.

This study identifies new functionally and molecularly distinct multipotent progenitors in adult human hematopoiesis using multiomic single cell analyses and functional assays.

The paper defines human HSPC subsets and differentiation trajectories using surface proteins and functional regulators, validated by epigenetic analysis and clonal assays.

This study identifies ASXL1 truncating mutations as a driver of leukemic resistance to T cell attack and proposes EZH2 inhibition as a therapeutic strategy to overcome this resistance.

This study identifies convergent epigenetic evolution in AML relapse using ATAC-seq and mtscATAC, showing that epigenetic changes drive relapse independent of genetic mutations.

The paper introduces TRACE-Seq, a method to track clonal reconstitution of gene-targeted human hematopoietic stem cells using barcoded AAV6 donor templates in immunodeficient mice.