Plant BiologyThe Plant JournalPeer-reviewed
Antisense transcription from a neighboring gene interferes with the expression of mNeonGreen as a functional in vivo fluorescent reporter in the chloroplast of Chlamydomonas reinhardtii.
Axel Navarrete, Bernardo Pollak
The paper develops a chloroplast codon-optimized mNeonGreen fluorescent reporter in Chlamydomonas reinhardtii, identifies antisense transcription interference, and improves reporter expression through regulatory sequence engineering.