Alexander N. Volkov

Multivalent ubiquitin recognition regulates multiple stages of Tau self-assembly

This study identifies multivalent ubiquitin recognition as a regulator of Tau self-assembly stages, showing ubiquitin's role in modulating condensate formation and aggregation.

Interplay of stability and dynamics in the optimization of a highly proficient de novo enzyme

This study identifies features contributing to catalytic efficiency in a de novo enzyme through computational design and directed evolution, showing how stability and dynamics interplay in enzyme optimization.

Insights into the recruitment of the H3K4me3 reader Spp1 by the meiotic double-strand break protein Mer2

This study investigates how Mer2 recruits Spp1 to form complexes involved in meiotic DSB machinery through structural and biochemical analysis.

Binding of long non-coding RNAs within the androgen receptor N-terminal tail modulates interdomain communication and liquid-liquid phase separation

This study identifies the RNA-binding region of the androgen receptor and shows how lncRNA binding modulates interdomain communication and phase separation, contributing to ligand-independent AR signaling in prostate cancer.

Emergence of specific binding and catalysis from a designed generalist binding protein

This study shows how a designed generalist binding protein can evolve into specific functions like a fluorophore binder and an efficient enzyme through weak, promiscuous interactions.

Ionic strength modulates structural disorder and protein oligomerization in the marginally disordered Phd transcription factor

This paper shows how ionic strength affects the structural disorder and oligomerization of the Phd transcription factor, revealing its ability to function as a conformational rheostat.

Negative design enables cell-free expression and folding of designed transmembrane β-Barrels

The paper demonstrates that negative design, which locally destabilizes β-strands, improves folding efficiency of synthetic transmembrane β-barrels in a cell-free system.

Recruitment of Mre11 to recombination sites during meiosis

This study identifies mechanisms by which Mre11 is recruited during meiosis to initiate recombination in budding yeast.

In silico design of stable single-domain antibodies with high affinity

This paper introduces EvolveX, a computational pipeline for designing stable single-domain antibodies with high affinity, demonstrating improved binding affinity to human targets.

Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes

The paper presents AlphaFold structures of Rec114-Mei4 and Mer2 complexes, showing their conserved structure across eukaryotes and DNA-binding properties.