Lorenzo Olivi

This study investigates how titration of an active DnaA variant controls DNA replication in E. coli under different growth conditions, revealing limits and design principles for synthetic replication systems.

This study investigates DnaA titration on the E. coli chromosome and shows it controls free DnaA levels in a growth rate-dependent manner, preventing re-initiation during slow growth.

The paper uses live-cell imaging to compare the target search efficiency of Cas9 and Cas12a variants, finding that LbdCas12a is faster and more efficient than SpydCas9.

The paper constructs a synthetic RNA polymerase operon in E. coli, showing that adaptive evolution can restore growth after deleting native genes.