Da Zhou

The paper investigates the reliability of mitochondrial DNA variants as lineage markers, finding that most are pre-existing heteroplasmy rather than de novo mutations, and introduces a score to identify reliable markers.

This study develops mathematical models to explore how chemotherapy and immune checkpoint blockade (ICB) combination therapy affects heterogeneous tumors, revealing chemotherapy's role in reducing antigenic heterogeneity and enhancing ICB efficacy through immune recovery.

scPhyloX infers tissue development and tumor evolution dynamics using single-cell phylogenetic trees, revealing novel insights into stem cell overshoot, hematopoietic progenitor clonal expansion, and colorectal tumor subclonal selection.

The paper introduces PhyloVelo, a method that uses monotonically expressed genes to reconstruct cell fate transitions, outperforming existing approaches in embryo development and tumor evolution analysis.