Robert Tampé

The paper identifies ionic locks in ABC transporters that govern directionality through kinetic asymmetry in ATP binding and hydrolysis.

The paper reveals calreticulin mediates glycan-dependent transfer of MHC I from TAPBPR to tapasin, essential for MHC I recycling and quality control.

This study uses single-molecule FRET to analyze ATP-driven conformational dynamics of TmrAB, revealing hidden intermediates and long ATP-bound states.

The paper uses Bayesian Markov models to explain asymmetric ATP hydrolysis kinetics in ABCE1 without requiring direct cooperativity between nucleotide-binding sites.

The paper uses single-molecule FRET to show ATP binding alone drives peptide translocation by TmrAB without Mg2+.

This paper reveals how the HCMV protein US6 inhibits the peptide-loading complex by altering TAP dynamics, providing insights into viral immune evasion mechanisms.

This paper introduces a method to insert synthetic peptides into proteins in eukaryotic cells to enable chemical modifications and non-canonical amino acids, validated using GFP, NaV1.5, and P2X2 receptors.

This paper describes a method for delivering large protein complexes into cells using DNA nanostructures and microfluidic squeezing, demonstrating delivery into HeLa cells and potential applications in immunotherapy.

This paper introduces fluorobodies, engineered nanobodies with bright fluorophores, enabling high-precision live-cell imaging of endogenous proteins with low background and toxicity.