Zhengliang L. Wu

The paper presents methods for glycan fingerprinting of therapeutic antibodies, identifying specific glycan structures on NISTmAb.

The paper detects substrate glycans of fucosyltransferases using fluorescent fucose incorporation to study their specificity and interactions with sialylation.

The paper uses direct fluorescent glycan labeling to image N- and O-glycans on HeLa cells, revealing distinct distribution patterns of Core-1 O-glycans, N-glycans, and sialyl T antigen.

The paper develops a method to specifically image glycan structures like HS and T/Tn antigens using glycosyltransferases and click chemistry in mesenchymal stem cells and HUVEC cells.