Author

Afsar Ali Mian

Aga Khan UniversityORCID 0000-0002-1995-7553Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Molecular Biology· 2 Cancer Biology· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:human· 3 Assay:CRISPR screen· 2 Research Focus:drug response / drug discovery· 2 Research Focus:gene regulatory network inference· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:cell type annotation· 1 Research Focus:differential expression· 1

Frequent Co-Authors

Irfan HussainiD· 3 shared Ambrin FatimaiD· 2 shared Fawad Ur RehmaniD· 2 shared Hijab ZahraiD· 2 shared Kainaat Mumtaz· 2 shared Maliha Javed· 2 shared Muhammad Jameel· 2 shared Oliver Gerhard OttmanniD· 2 shared

Activity by Year

2018-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 34 mutual co-author links

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3 shared papersMolecular Biology

Irfan HussainORCID

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

Cell BiologybioRxivPreprintSep 24, 2026

Prime Editing Corrects the HBB Codon 8/9 (+G) Mutation in Patient-Derived Induced Pluripotent Stem Cells and Restores β-Globin Expression in iPSC-Derived Erythroid Cells

Irfan Hussain, Kainaat Mumtaz, Maliha Javed…·Afsar Ali Mian

This study uses prime editing to correct the HBB c.27dupG mutation in patient-derived iPSCs and restores β-globin expression in erythroid cells.

DOI 10.64898/2026.09.22.753453

humanCRISPR screen

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Molecular BiologybioRxivPreprintNov 13, 2024

A Rapid, Cost-Effective Method for High-Yield DNA Purification from PCR Products and Agarose Gels.

Susheel Fatima, Maliha Javed, Hijab Zahra…·Afsar Ali Mian

The paper develops a cost-effective in-house DNA purification protocol for PCR products and agarose gels, showing improved clarity and purity compared to commercial kits.

DOI 10.1101/2024.11.13.623516

E. coliDNA methylation

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Molecular BiologybioRxivPreprintNov 28, 2023

Prioritization of The Zinc finger domain within BCL11A gene by the Amelioration capability of hemoglobinopathies using CRISPR-Cas9 technology

Kainaat Mumtaz, Irfan Hussain, Fizza Iftikhar…·Afsar Ali Mian

This study uses CRISPR-Cas9 to investigate BCL11A zinc finger domains' role in gamma-globin regulation and hemoglobinopathy treatment, finding Znf4 deletion increases HbF levels.

DOI 10.1101/2023.11.28.568974

humanCRISPR screen

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Cancer BiologybioRxivPreprintNov 7, 2018

Subtractive Interaction Proteomics Reveal a Network of Signaling Pathways Activated by an Oncogenic Transcription Factor in Acute Myeloid Leukemia

Nathalie Guillen, Maria Wieske, Andreas Otto…·Claudia Chiriches

This study uses subtractive interaction proteomics to identify signaling pathways activated by the t(6;9)-DEK/CAN oncogene in AML, revealing potential therapeutic targets.

DOI 10.1101/464958

humanproteomics

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