Yi Liu

This paper introduces an enhanced SCas12a assay (SCas12aV2) for amplification-free detection of highly structured RNA, showing improved sensitivity and specificity for RNA and clinical samples.

This paper develops a split CRISPR-Cas12a system for sensitive, selective, and multiplexed detection of RNA and DNA without reverse transcription, achieving low detection limits and improved specificity.

This paper presents a method to directly produce and purify CRISPR/Cas RNP from E. coli in a single step using an ultra-high-affinity CL7/Im7 system, achieving high stability and genome editing efficiency.

The paper describes a method to directly prepare Cas9 RNP from E. coli for PCR-free DNA assembly with high fidelity.